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Clinical and histological findings in experimental autoimmune encephalomyelitis (EAE) after mitochondrial transplantation. (A) Clinical scores assessed in saline-injected (vehicle) and mitochondria-injected mice throughout the course of EAE. Data are shown as representative results (mean ± SEM) from three independent experiments (n=5 per group) (B) Concentrations of MOG-specific IgG in serum quantified by enzyme-linked immunosorbent assay. (C) Mitochondrial ROS levels measured by MitoSOX fluorescence analyzed in splenocyte populations using ex vivo flow cytometry. (D) Spinal cord sections subjected to H&E (hematoxylin and eosin) staining to assess inflammatory cell infiltration and LFB (Luxol Fast Blue) staining to evaluate demyelination666. Enlarged insets (magnified views) are provided to highlight perivascular infiltration and myelin loss. * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Frontiers in Immunology

Article Title: Mitochondrial transplantation ameliorates experimental autoimmune encephalomyelitis by modulating the Th17/Treg balance and restoring metabolic homeostasis

doi: 10.3389/fimmu.2026.1698136

Figure Lengend Snippet: Clinical and histological findings in experimental autoimmune encephalomyelitis (EAE) after mitochondrial transplantation. (A) Clinical scores assessed in saline-injected (vehicle) and mitochondria-injected mice throughout the course of EAE. Data are shown as representative results (mean ± SEM) from three independent experiments (n=5 per group) (B) Concentrations of MOG-specific IgG in serum quantified by enzyme-linked immunosorbent assay. (C) Mitochondrial ROS levels measured by MitoSOX fluorescence analyzed in splenocyte populations using ex vivo flow cytometry. (D) Spinal cord sections subjected to H&E (hematoxylin and eosin) staining to assess inflammatory cell infiltration and LFB (Luxol Fast Blue) staining to evaluate demyelination666. Enlarged insets (magnified views) are provided to highlight perivascular infiltration and myelin loss. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The levels of MOG-specific antibodies were then detected using the Mouse IgG2 ELISA Quantitation Set (A90-107P, Bethyl Laboratories, Montgomery, TX, USA) according to the manufacturer’s instructions.

Techniques: Transplantation Assay, Saline, Injection, Enzyme-linked Immunosorbent Assay, Fluorescence, Ex Vivo, Flow Cytometry, Staining